Identification of Lactic Acid Bacteria Isolated from Local Commercial Yahe (Fermented Caridean-Shrimp) Products

Say Sophakphokea, Rith Sokuncharya, Norng Chakriya, Ang Vichheka, Chheun Malyheng, Ly Sokheng, Prom Kimheang, C. Rithy, C. Chim
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Abstract

Fermentation was used since ancient times as an easy method of food preservation, which also maintains and/or improves the nutritional and sensory properties of food. A research as aimed at identifying strain of lactic acid bacteria (LAB) from fermented caridean-shrimp, which properties suitable for starter cultures in food fermentation. A total of 18 LAB stains were obtained from ten different samples, in each sample consisted of commercial LAB strain that isolated from ten samples of caridean-shrimp. The LAB strains from ten samples were screened for resistance to biological barriers (acid and bile salts), and the three most promising strains were selected. The three bacteria strains were isolated from samples of caridean[1]shrimp and were characterized by the API 50 CHL system of identification. Three lactic acid bacteria species were identified and included Lactobacillus plantarum, and Lactobacillus acidophilus. Strain Y’11b,2, Y’11e,2, Y’85,1, which showed probiotic characteristics reducing cell growth of cancer, could be suitable as a starter culture for food fermentation because of its strong acid production and high acid tolerance. This is the first report to describe bacteria, isolated from caridean[1]shrimp, Lactobacillus Plantarum (Y’11b,2, Y’11e,2) and Lactobacillus acidophilus (Y’85,1) which have the probiotic characteristics and the acid tolerance needed for its use as a starter culture in food fermentation.
地方商业产虾发酵产品中乳酸菌的鉴定
自古以来,发酵就被用作一种简单的食物保存方法,它还能保持和/或改善食物的营养和感官特性。本研究旨在鉴定一株适合用于食品发酵发酵剂的乳酸菌。从10个不同的样品中共获得18个LAB染色,每个样品由从10个caridean-shrimp样品中分离的商业LAB菌株组成。从10个样品中筛选乳酸菌对生物屏障(酸和胆盐)的抗性,筛选出3株最有希望的乳酸菌。这3株菌株均从虾蛄[1]中分离得到,采用API 50 CHL体系进行鉴定。鉴定出三种乳酸菌,包括植物乳杆菌和嗜酸乳杆菌。菌株Y ' 11b,2, Y ' 11e,2, Y ' 85,1具有降低肿瘤细胞生长的益生菌特性,产酸能力强,耐酸能力强,适合作为食品发酵发酵剂。这是第一次报道从caridean[1]虾、植物乳杆菌(Y ' 11b,2, Y ' 11e,2)和嗜酸乳杆菌(Y ' 85,1)中分离出的细菌,它们具有益生菌特性和耐酸性,可以用作食品发酵的发酵剂。
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