Estudo da hidrólise ácida e enzimática da borra de café como alternativa de uso deste resíduo

Ciência e Tecnologia dos Alimentos–Volume 2 Pub Date : 1900-01-01 DOI:10.36229/978-85-7042-120-3.cap.13

D. Angeli, I. C. M. Andrei, A. Dekker, Claudio Takeo Ueno, L. F. Dias

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Abstract

The soluble coffee industry produces a large amount of coffee grounds waste with many applications. The aim of this study was to assess the effectiveness of acid hydrolysis (HCl) and enzymatic (Viscozyme L) of coffee grounds waste. The acid hydrolysis was performed in different conditions of temperature and pressure using water bath and autoclave. The optimization of the parameters of enzymatic hydrolysis (pH, time, temperature) were determined by central composite design (CCRD) using as enzyme substrate, Whatman no1 paper. The best conditions obtained for hydrolysis of cellulose filter paper were pH 4.0 at 45 °C / hour, equivalent to 50 FBG (β-glucanase fungal) but the coffee grounds enzymatically hydrolyzed under these conditions, did not provide satisfactory results. Thus, it was decided by a combination of enzymatic hydrolysis followed by acid hydrolysis using hydrochloric acid (120 °C / 2 h) to yield a significant amount of glucose compared to acid hydrolysis. PALAVRAS-CHAVE: Resíduo lignocelulósico, Viscozyme L, Planejamento experimental.

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研究咖啡渣的酸水解和酶水解作为这一废物的替代用途

可溶咖啡工业产生大量的咖啡渣废物，有许多用途。本研究的目的是评估酸水解(HCl)和酶(粘酶L)对咖啡渣废物的有效性。采用水浴和高压灭菌器在不同温度和压力条件下进行酸水解。以Whatman no1号纸为酶底物，采用中心复合设计(CCRD)确定酶解参数(pH、时间、温度)的优化。纤维素滤纸的最佳水解条件是pH为4.0，温度为45℃/小时，相当于50 FBG (β-葡聚糖酶真菌)，但在此条件下酶解的咖啡渣并不能提供令人满意的结果。因此，与酸水解相比，酶水解和盐酸酸水解(120°C / 2 h)的结合决定了葡萄糖的产量。PALAVRAS-CHAVE: Resíduo lignocelulósico，粘酶L, Planejamento实验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Ciência e Tecnologia dos Alimentos–Volume 2

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