Flow of /spl lambda/-DNA in microfluidic devices

Abstract

This research seeks to understand the behavior of biological macromolecules in microfluidic devices. Epifluorescence microscopy was used to observe the conformation of /spl lambda/-DNA macromolecules during flow through sudden expansions, sudden contractions, straight channels, and a valve. The authors' analysis indicates that both the flow patterns within the device and the device geometry can influence macromolecular structure and stability. Utilization of this type of analysis in the design of microsystems for biochemical processing is instrumental for maintaining molecular stability and for ensuring accuracy of sample analysis.