Lactoferrin, Isolation,Purification and Antimicrobial Effects

Abstract

Lactoferrin (Lf) is an 80 kDa iron-binding glycoprotein with multifunctional properties and useful for clinical and commercial applications. Currently, Lf has some advantages including, immune system modulation, antibacterial activity and as antioxidant in infant and adult of human as well as animal health. In the present study, antibacterial activity of Lf has been scrutinized after isolation and purification from cow’s milk colostrums against Pseudomonas aeroginosa. Lf purified using CMsephadex C50, a cation exchange chromatography. Bacterial samples were isolated from scald patients and microbial activity was confirmed by biochemical tests. Different concentration of Lf (0, 200, 400, 500, 600 and 700 μg/ml) treated on Pseudomonas colonies as well as E.coli( DH5α) as positive control for two days. Our result indicated LF was effective on Pseudomonas growth and the least and the inset inhibitory concentration were 400,and 700 μg/ml, respectively. The result showed that The Lf has more strong effect than other previous studies. 