F. Brandi, D. Heck, Thiago Costa Ferreira, W. Bettiol
{"title":"Commercial formulations of Bacillus spp. for sugarcane pineapple disease control and growth promotion","authors":"F. Brandi, D. Heck, Thiago Costa Ferreira, W. Bettiol","doi":"10.1590/s0100-204x2018001200003","DOIUrl":null,"url":null,"abstract":"Abstract: The objective of this work was to evaluate the commercial formulations of Bacillus subtilis QST-713 and Bacillus pumilus QST-2808 on mycelial growth inhibition of Thielaviopsis paradoxa, for the control of the pineapple disease and growth promotion in sugarcane. The inhibition of mycelial growth was evaluated in paired culture tests. Bacillus isolates were transferred to Petri dishes 48 hours before the pathogen, and the zone of inhibition was determined. The direct effect of the autoclaved commercial formulations at 0, 0.001, 0.01, 0.1, 1.0, and 10.0% on the inhibition of pathogen mycelial growth was evaluated. Bacillus spp. potential in controlling the disease and promoting plant growth was evaluated in assays on plant development and disease severity, both under controlled conditions and in the field. Bacillus isolates inhibited pathogen mycelial growth; however, the isolates did not control the disease effectively in the growth chamber and in the field. In the field assay without infestation by the pathogen, all treatments with biocontrol agents and fungicide increased the yield of cane stalks and sugar per hectare, compared with the control. In the assay with infestation by the pathogen, the B. pumilus-based product (2.0 L ha-1) and fungicide differed from the control only for the variable number of tillers.","PeriodicalId":185886,"journal":{"name":"Pesquisa Agropecuária Brasileira","volume":"44 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesquisa Agropecuária Brasileira","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/s0100-204x2018001200003","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Abstract: The objective of this work was to evaluate the commercial formulations of Bacillus subtilis QST-713 and Bacillus pumilus QST-2808 on mycelial growth inhibition of Thielaviopsis paradoxa, for the control of the pineapple disease and growth promotion in sugarcane. The inhibition of mycelial growth was evaluated in paired culture tests. Bacillus isolates were transferred to Petri dishes 48 hours before the pathogen, and the zone of inhibition was determined. The direct effect of the autoclaved commercial formulations at 0, 0.001, 0.01, 0.1, 1.0, and 10.0% on the inhibition of pathogen mycelial growth was evaluated. Bacillus spp. potential in controlling the disease and promoting plant growth was evaluated in assays on plant development and disease severity, both under controlled conditions and in the field. Bacillus isolates inhibited pathogen mycelial growth; however, the isolates did not control the disease effectively in the growth chamber and in the field. In the field assay without infestation by the pathogen, all treatments with biocontrol agents and fungicide increased the yield of cane stalks and sugar per hectare, compared with the control. In the assay with infestation by the pathogen, the B. pumilus-based product (2.0 L ha-1) and fungicide differed from the control only for the variable number of tillers.
摘要:本研究旨在评价枯草芽孢杆菌QST-713和短小芽孢杆菌QST-2808两种商品制剂对异氏Thielaviopsis的菌丝生长抑制作用,以防治菠萝病和促进甘蔗生长。在配对培养试验中评估了对菌丝生长的抑制作用。将分离的芽孢杆菌在病原菌产生前48小时转移到培养皿中,确定抑菌区。研究了0、0.001、0.01、0.1、1.0和10.0%的商业配方对病原菌菌丝生长的直接抑制作用。通过控制条件下和田间的植物发育和病害严重程度试验,评价了芽孢杆菌控制病害和促进植物生长的潜力。分离芽孢杆菌抑制病原菌菌丝生长;然而,分离株在生长室内和田间均不能有效控制该病。在无病原菌侵染的田间试验中,与对照相比,所有生物防治剂和杀菌剂处理均提高了每公顷甘蔗茎和糖的产量。在有病原菌侵染的试验中,只有分蘖数不同,以小圆圆杆菌为基础的产物(2.0 L ha-1)和杀菌剂与对照不同。