Application of CRISPR/Cas-based Biosensing Platform in Molecular Diagnostics

Abstract

The CRISPR/Cas system is widely used in genome editing. The CRISPR/Cas system can accurately cut and recognize DNA and RNA sequences. In the process of recognizing target sequences, the CRISPR/Cas system can not only recognize the target sequence to start the targeted cleavage activity, and also show non-specific cleavage activity in DNA, RNA molecules and other single strands, add fluorescent group reporter nucleic acid molecules to the reaction system, and observe through fluorescent signals to the location of the target nucleic acid molecule, and with the help of the different cutting preferences of the Cas protein, it can also detect multiple target molecules at the same time.