Fiber optic bio-sniffer (biochemical gas sensor) using UV-LED light for monitoring ethanol vapor with high sensitivity & selectivitiy

2009 IEEE Sensors Pub Date : 2009-10-01 DOI:10.1109/ICSENS.2009.5398336

H. Kudo, K. Miyajima, D. Takahashi, T. Arakawa, H. Saito, K. Mitsubayashi, M. Sawai

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引用次数: 3

Abstract

A fiber optic bio-sniffer (biochemical gas sensor) for alcohol gas monitoring with high sensitivity and high selectivity was fabricated and tested. The bio-sniffer is a gas sensor that uses molecular recognition of enzyme to improve selectivity. Usually, enzyme loses activity in the gas phase. Applying a flow-cell with a gas-intake window to the sensing probe, enzyme immobilized at the sensing region was kept in the sufficient wet condition to maintain activity. The bio-sniffer measures ethanol (EtOH) vapor by measuring fluorescence of nicotinamide adenine dinucleotide (NADH), which is produced by enzymatic reaction at the flow-cell. In order to construct a simplified system suitable for on-site applications, a high-intensity ultraviolet light emitting diode (UV-LED) was utilized as an excitation light. Owing to low power consumption comparing with previous light sources, the bio-sniffer was considered to be suitable for laptop applications such as on-site monitoring. According to the characterization, the bio-sniffer for was useful for continuous alcohol monitoring and showed high selectivity. The calibration range was 0.30–300 ppm which is suitable for evaluation of capacity to metabolize alcohol.

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光纤生物嗅探器(生化气体传感器)采用UV-LED光监测乙醇蒸气，具有高灵敏度和选择性

研制了一种高灵敏度、高选择性的用于酒精气体监测的光纤生物嗅探器(生化气体传感器)。生物嗅探器是一种利用酶的分子识别来提高选择性的气体传感器。通常，酶在气相中失去活性。在传感探针上应用带进气窗的流动池，将固定在传感区域的酶保持在足够潮湿的条件下以保持活性。生物嗅探器通过测定在流动细胞中酶促反应产生的烟酰胺腺嘌呤二核苷酸(NADH)的荧光来测定乙醇(EtOH)蒸气。为了构建适合现场应用的简化系统，采用高强度紫外发光二极管(UV-LED)作为激发光源。由于与以前的光源相比功耗低，生物嗅探器被认为适合于现场监测等笔记本电脑应用。实验结果表明，该生物嗅探器具有较高的选择性，可用于酒精的连续监测。校准范围为0.30-300 ppm，适用于评估酒精代谢能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2009 IEEE Sensors

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