Effect of Bambara groundnut (Vigna subterranea) consumption on biomarkers of oxidative stress in alloxan-induced diabetic Wistar rats

Research Journal of Food Science and Nutrition Pub Date : 2019-06-30 DOI:10.31248/RJFSN2019.066

F. Adedeji, G. Adeniyi, E. Omenna, O. T. Olanipekun

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引用次数: 1

Abstract

This study investigated the effect of consumption of diet supplemented with Bambara-groundnut (BGN) on some biomarkers of oxidative stress in diabetic induced rats. A total of 24 apparently healthy adult albino wistar rats were assembled and divided into 4 groups A-D; group A (Non diabetic rats, fed on normal diet), B (Diabetic rats fed on normal diet), C (Diabetic rats fed on 10% Bambara-groundnut diet), D (Diabetic rats fed on Nitrogen free diet) with each group consisting of 6 rats respectively. After 4 weeks of feeding, rats were sacrificed and blood collected into heparin container and used for laboratory analysis. Superoxide dismutase (SOD), glutathione peroxidase (GSHPER), alkaline phosphatase (ALP), alanine amino transferase (ALT), aspartate transaminase (AST) were assayed for using standard laboratory methods. The result showed that Group C rats had the lowest post-feeding glucose concentration (75 mg/dl). AST, ALT, and ALP were elevated in diabetic induced rats; but, treatment with Bambara-groundnut diet reversed the parameters close to control. The highest levels of SOD and GSHPER were found in group C rats while the group B rats had the highest oxidative stress. It can be concluded that Bambara-groundnut diet modulated and ameliorated the oxidative stress in diabetic rats.

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食用班bara groundnut (Vigna subterranea)对四氧胺诱导的糖尿病Wistar大鼠氧化应激生物标志物的影响

本研究探讨了添加班巴拉花生(BGN)的饮食对糖尿病大鼠氧化应激一些生物标志物的影响。选取表面健康的成年白化wistar大鼠24只，分为A- d组;A组(非糖尿病大鼠，喂食正常饲料)、B组(喂食正常饲料的糖尿病大鼠)、C组(喂食10%班巴拉花生饲料的糖尿病大鼠)、D组(喂食无氮饲料的糖尿病大鼠)，每组6只。饲养4周后，处死大鼠，取血入肝素容器，用于实验室分析。采用标准实验室方法检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPER)、碱性磷酸酶(ALP)、丙氨酸氨基转移酶(ALT)、天冬氨酸转氨酶(AST)。结果显示，C组大鼠饲后葡萄糖浓度最低(75 mg/dl)。糖尿病大鼠AST、ALT、ALP升高;但是，班巴拉花生饮食使这些参数逆转，接近对照组。SOD和GSHPER以C组最高，氧化应激以B组最高。由此可见，班巴拉花生对糖尿病大鼠氧化应激有调节和改善作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Research Journal of Food Science and Nutrition

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