Generation and Selective Retrieval of Micro Droplets in an Array for Micro-PCR using a Glass-Silicon-Glass Chip

Abstract

In this study, we fabricate a glass-silicon-glass chip for the generation of a water-droplet array. Using this array, we succeeded in performing polymerase chain reaction (PCR) within the hundreds of trapped water droplets. Then, we selectively retrieve a specific PCR droplet with a bubble formed by heating an aluminum micro pad with an infrared laser.