MORPHOGENESIS IN VITRO IN BARLEY CALLUS: INFLUENCE OF NAPHTYLPHTHALAMIC ACID

Abstract

Morphogenesis in vitro in barley calli of the cv. Steptoe and its ABA-deficient mutant AZ34 under the influence of an inhibitor of polar auxin transport, N-1-naphthylphthalamic acid (NPA), was studied by light microscopy The formation of «polymeric» embryoids (polyembryoids), characterized by the presence of multiple, often fused (fasciated) organs in varying degrees, in calli of cv. Steptoe and the formation of fasciated roots in calli of AZ34 were revealed. The distribution of IAA in calli was studied by the immunolocalization method. It was found that the most intense staining for IAA in both genotypes was found in fasciated roots. The important role of the polar transport of auxins in the regulation of plant morphogenesis in vitro has been confirmed.