OPTIMIZATION OF THE PROCEDURE OF LACTATE AND PYRUVATE DETERMINATION IN BLOOD SERUM USING A BIOSENSOR SYSTEM

Sensor Electronics and Microsystem Technologies Pub Date : 2018-09-11 DOI:10.18524/1815-7459.2018.3.142042

I. Kucherenko, Ya. V. Topolnikova, D. V. Knyzhnykova, O. Soldatkin, С. Кучеренко, Я. В. Топольникова, Д. В. Книжникова, О. О. Солдаткин

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Abstract

The purpose of this work was to optimize the procedure for simultaneous determination of concentrations of lactate and pyruvate in human serum using a biosensor system. The system consisted of two amperometric biosensors based on pyruvate oxidase and lactate oxidase, respectively, that were connected to one measuring circuit and simultaneously operated in a single measuring cell. In the work, the dependence of the responses of the biosensor system on the dilution of serum in the working cell was tested. The reproducibility of the responses and the change in the calibration curves of the biosensor system when working with serum were studied. The concentrations of lactate, pyruvate and their ratio in blood serum samples were determined and a high correlation of the results with the data of the control method was shown. It is proved that the proposed biosensor system is suitable for the rapid determination of lactate, pyruvate and their ratio and can be used in the medical diagnostics of the relevant diseases.

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生物传感器系统测定血清乳酸和丙酮酸的方法优化

本工作的目的是优化使用生物传感器系统同时测定人血清中乳酸和丙酮酸浓度的方法。该系统由两个分别基于丙酮酸氧化酶和乳酸氧化酶的安培生物传感器组成，它们连接到一个测量电路中，在单个测量池中同时工作。在工作中，测试了生物传感器系统对工作细胞中血清稀释度的依赖性。研究了该生物传感器系统在血清中工作时的可重复性和校准曲线的变化。测定了血清样品中乳酸、丙酮酸的浓度及其比值，结果与对照法的数据有较高的相关性。实验证明，该生物传感器系统适用于乳酸、丙酮酸及其比值的快速测定，可用于相关疾病的医学诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Sensor Electronics and Microsystem Technologies

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