Observation of carbon nanotubes in water by supplying fluorescent reagent with porous structured PDMS supports

F. Arai, N. Inomata, R. Ookawara, Y. Yamanishi, Yu-Ching Lin
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Abstract

We have demonstrated a visualization method of carbon nanotubes (CNTs) in water with fluorescent microscopy by using fluorescent dye background conditions that display a reversal contrast. We have successfully observed CNTs under a constant excitation light for more than ten minutes. We have developed novel chip system to supply fluorescence dye with appropriate concentration. The salt reaching method has been applied to PDMS (poly-dimethyl siloxane) in order to obtain porous structures. We have found that this porous structure provides homogeneous concentration of fluorescence dye by putting it in water and found out porous-PDMS support with larger porous gives a higher and steady concentration. The fine control of the concentration of the fluorescence dye in the microchannel has been achieved with a controlled speed of flow and mixture by means of a micro magnetic stirrer. Dielectrophoretic force was used for CNTs deposition onto electrodes that consist of transparent conductive film. It was confirmed that the proposed microfluidic system controls the concentration of fluorescent dye for a long term observation in water and is useful to apply CNTs to several biological application fields.
用多孔结构的PDMS支撑荧光试剂观察水中碳纳米管
我们展示了一种用荧光显微镜观察水中碳纳米管(CNTs)的可视化方法,该方法使用荧光染料背景条件显示反向对比。我们已经成功地在恒定激发光下观察了十多分钟的CNTs。我们开发了一种新颖的芯片系统来提供合适浓度的荧光染料。将盐到达法应用于PDMS(聚二甲基硅氧烷)中以获得多孔结构。我们发现这种多孔结构通过将荧光染料置于水中可以提供均匀的浓度,并且多孔的pdms载体具有更高且稳定的浓度。利用微磁搅拌器,通过控制流动速度和混合速度,实现了对微通道中荧光染料浓度的精细控制。介质电泳力用于将碳纳米管沉积到由透明导电膜组成的电极上。实验结果表明,该微流控系统能够控制水中荧光染料的浓度,实现对水中荧光染料的长期观察,可将碳纳米管应用于多个生物领域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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