Response surface methodology based optimization of sorbitol production via solid state fermentation process

Q2 Engineering
Zuriana Sidi Ahmad, Mimi Sakinah Abdul Munaim
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引用次数: 4

Abstract

To optimize sorbitol production through solid state fermentation (SSF) process using response surface methodology (RSM), the central composite design (CCD) was employed in this study in order to minimize the number of experiments needed for the determination of the best combination of parameters for the process optimization. The raw material used in this study was Meranti wood sawdust (MWS) obtained from Gambang Sawmill (M) Sdn Bhd, Gambang Kuantan, Pahang. The type of bacteria used for the study was Lactobacillus plantarum (BAA 793; NCIMB 8826), purchased from the America Type Culture Collection (ATCC). The physical and chemical pretreatment methods were used in the study to recover cellulose from the MSW, followed by the enzymatic hydrolysis process to produce glucose. The last phase of this work was the fermentation process using SSF to convert the produced glucose to sorbitol; this is the section of the study where the parameters were optimized using the RSM. The result of the RSM studies showed that the interactions between moisture content and temperature had a very significant effect with an F value of <0.0001. The highest yield of sorbitol (29.0625 g/L) was obtained when using 50% of moisture content, at a temperature of 35 °C for 10 h of fermentation time. The RSM helped in the optimization of the effective parameters as well as to analyze the interaction between the parameters.

基于响应面法的固体发酵山梨醇生产工艺优化
为了利用响应面法(RSM)对固态发酵(SSF)工艺进行优化,本研究采用中心组合设计(CCD),以最大限度地减少实验次数,确定工艺优化的最佳参数组合。本研究使用的原料是来自彭亨州甘邦关丹甘邦锯木厂(M) Sdn Bhd的莫兰蒂木屑(MWS)。用于研究的细菌类型为植物乳杆菌(baa793;NCIMB 8826),购自美国字体文化收藏(ATCC)。本研究采用物理和化学预处理方法从城市生活垃圾中回收纤维素,然后进行酶解生产葡萄糖。本工作的最后一个阶段是利用SSF将产生的葡萄糖转化为山梨醇的发酵过程;这是研究中使用RSM优化参数的部分。RSM研究结果表明,水分含量与温度之间的相互作用具有非常显著的影响,F值为<0.0001。当温度为35 ℃,发酵时间为10 h,含水量为50%时,山梨糖醇的产率最高,为29.0625 g/L。RSM有助于优化有效参数,并分析参数之间的相互作用。
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来源期刊
Engineering in Agriculture, Environment and Food
Engineering in Agriculture, Environment and Food Engineering-Industrial and Manufacturing Engineering
CiteScore
1.00
自引率
0.00%
发文量
4
期刊介绍: Engineering in Agriculture, Environment and Food (EAEF) is devoted to the advancement and dissemination of scientific and technical knowledge concerning agricultural machinery, tillage, terramechanics, precision farming, agricultural instrumentation, sensors, bio-robotics, systems automation, processing of agricultural products and foods, quality evaluation and food safety, waste treatment and management, environmental control, energy utilization agricultural systems engineering, bio-informatics, computer simulation, computational mechanics, farm work systems and mechanized cropping. It is an international English E-journal published and distributed by the Asian Agricultural and Biological Engineering Association (AABEA). Authors should submit the manuscript file written by MS Word through a web site. The manuscript must be approved by the author''s organization prior to submission if required. Contact the societies which you belong to, if you have any question on manuscript submission or on the Journal EAEF.
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