Photodynamic effect of different aluminum and zinc phthalocyanines on isolated nerve cell

Current Research on Laser Use in Oncology Pub Date : 1999-12-30 DOI:10.1117/12.375260

A. Uzdensky, A. Zhavoronkova, O. Dergacheva, V. Derkacheva

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引用次数: 2

Abstract

The photodynamic effects of sulphonated zinc (ZnPcS2, ZnPcS3 and ZnPcS4) and aluminum (AlPcS3) phthalocyanines as well as phosphonated aluminum phthalocyanine on the firing of isolated crayfish mechanoreceptor neurons were studied. After 30 min photosensitization neurons were irradiated with He-Ne laser (632.8 nm, 0.3 W/cm2) and changes in neuron firing frequency were recorded. Neuron firing was found to be very sensitive to photodynamic impact and served as a sensitive indicator of cell photodamage. The dynamics of the neuron responses to photodynamic effects included stages of firing activation and/or inhibition prior to irreversible firing abolition and depended on the photosensitizer type and concentration. The comparison of the dependencies of neuron lifetime on photosensitizer concentrations showed that the most effective photosensitizer was ZnPcS2. High photodynamic efficiencies of phthalocyanines was related with the weak dependence of photodynamic effect on sensitizer concentration indicating to the initiation about 3 secondary chain processes such as free radical membrane damage caused by photon absorption by one photosensitizer molecule.

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不同酞菁铝锌对离体神经细胞的光动力效应

研究了磺化锌(ZnPcS2、ZnPcS3和ZnPcS4)、铝(AlPcS3)酞菁以及膦化铝酞菁对离体小龙虾机械受体神经元放电的光动力学影响。光敏30 min后，用He-Ne激光(632.8 nm, 0.3 W/cm2)照射神经元，记录神经元放电频率的变化。神经元放电对光动力冲击非常敏感，是细胞光损伤的敏感指标。神经元对光动力效应的反应动力学包括在不可逆放电消除之前的放电激活和/或抑制阶段，并取决于光敏剂的类型和浓度。比较光敏剂浓度对神经元寿命的影响，发现ZnPcS2是最有效的光敏剂。酞菁的高光动力效率与光动力效应对光敏剂浓度的依赖性较弱有关，表明一个光敏剂分子吸收光子可引发自由基膜损伤等3个二级链过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Current Research on Laser Use in Oncology

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