Nano-injection system based on nanorobotic manipulations inside hybrid microscope

Abstract

This paper presents a novel nano-injection system based on nanorobotic manipulation inside hybrid microscope, which is combined with optical and environmental-scanning electron microscope (E-SEM). The hybrid microscope is designed to combine the optical microscope (OM) and E-SEM to realize biological specimen analysis by optical microscope image including fluorescent imaging, and nano-scale manipulation by E-SEM imaging. In this paper, we propose the nano-injection method based on nanorobotic manipulation inside the hybrid microscope. The nanoprobe has a sharp tip to insert the probe into biological sample. The probe is fabricated by focused ion beam (FIB) process. The Caenorhabditis elegans (C. elegans) is used as model organism to analysis various diseases in precisely and shot time. The nanoinjection technique is needed to transport fluorescent materials or specific biological organism into specific cell as in-vivo experiment. The proposed system is considered to be important as future nano-surgery system for life innovation using model organism.