Immunological methods for the quantitation of the industrially important bioleaching microorganisms.

C A Jerez, R Arredondo
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Abstract

To monitor the levels of Thiobacillus ferrooxidans in bioleaching operations, we have developed a specific and very sensitive dot-immunobinding assay. Polyclonal antisera against whole T. ferrooxidans cells was used, and the bacteria-antibody reaction was visualized by employing either 125I-labeled or peroxidase-conjugated protein A or 125I-labeled or peroxidase-conjugated goat anti-rabbit immunoglobulin G. A minimum of 10(3) cells per dot could be easily detected. Therefore, the method allows the sensitive, and specific simultaneous processing of numerous samples in a short time.

工业上重要的生物浸出微生物定量的免疫学方法。
为了监测生物浸出操作中氧化亚铁硫杆菌的水平,我们开发了一种特异性和非常敏感的点免疫结合试验。采用抗氧化t铁细胞的多克隆血清,并用125i标记或过氧化物酶结合的蛋白A或125i标记或过氧化物酶结合的山羊抗兔免疫球蛋白g观察细菌抗体反应,每个点至少可检测到10(3)个细胞。因此,该方法可以在短时间内对大量样品进行敏感、特异的同时处理。
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