Liquid-phase detection of biological targets with magnetic markers and high Tc SQUID

2013 IEEE 14th International Superconductive Electronics Conference (ISEC) Pub Date : 2013-07-07 DOI:10.1109/ISEC.2013.6604304

S. Uchida, Y. Higuchi, Y. Ueoka, T. Yoshida, K. Enpuku, S. Adachi, K. Tanabe, A. Tsukamoto

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引用次数: 1

Abstract

We developed a highly sensitive HTS SQUID system for liquid-phase detection of biological targets using Brownian relaxation of magnetic markers. The SQUID was made of a ramp-edge Josephson junction using RE123-based multilayer process, and it showed a flux noise of 7.5 micro flux-quantam/Hz1/2 in the white noise region. The 1/f noise was 14 micro flux-quantam/Hz1/2 at f = 1 Hz when the SQUID was operated with AC bias mode. Using the SQUID system, we detected a biological target called biotin. In the experiment, biotins were fixed to a large polymer bead with a diameter of 3.3 micron meter. Streptavidin-coated magnetic markers were used for the detection. The bound and unbound (free) markers were magnetically distinguished using the difference in their Brownian relaxation time, i.e., without using the washing process to separate them. The minimum detectable number of biotins was as low as 104, corresponding to a sensitivity of 4.8×10-19 mol/ml. This result indicates a high sensitivity of the developed method.

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磁性标记物和高Tc SQUID液相检测生物靶标

我们开发了一种高灵敏度的HTS SQUID系统，用于利用磁性标记物的布朗弛豫液相检测生物靶标。采用基于re123的多层工艺制备了斜边Josephson结，在白噪声区显示出7.5微通量量子/Hz1/2的通量噪声。在交流偏置模式下，f = 1 Hz时的1/f噪声为14微通量量子/Hz1/2。利用SQUID系统，我们检测到一种叫做生物素的生物靶标。在实验中，生物素被固定在一个直径为3.3微米的大聚合物珠上。采用链霉亲和素包被磁标记进行检测。结合和未结合(自由)标记是用它们的布朗弛豫时间的差异来磁性区分的，即不使用洗涤过程来分离它们。生物素的最低检测数低至104，相应的灵敏度为4.8×10-19 mol/ml。结果表明，该方法具有较高的灵敏度。

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2013 IEEE 14th International Superconductive Electronics Conference (ISEC)

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