Kimber L. Brenneman, B. Sen, M. Stroscio, M. Dutta
{"title":"Aptamer-based optical bionano sensor for mercury(II) ions","authors":"Kimber L. Brenneman, B. Sen, M. Stroscio, M. Dutta","doi":"10.1109/NMDC.2010.5652331","DOIUrl":null,"url":null,"abstract":"The detection of Hg2+ ions at the nanomolar level was achieved using a DNA aptamer as the molecular recognition element. The single stranded DNA sequence known as thrombin binding aptamer (TBA) was conjugated to a quantum dot and a gold nanoparticle to create the mercury-sensing probe. The quantum dot donor and gold nanoparticle acceptor allowed for optical detection via energy transfer using a spectrophotometer. The presence of the analyte caused a decrease in photoluminescence. The quenching efficiency at 500 nM Hg2+ ions with 10 nM aptamer probe was 62%. When the probe was exposed to a much higher concentration of Hg2+ ions, 824 µM, the photoluminescence was completely extinguished.","PeriodicalId":423557,"journal":{"name":"2010 IEEE Nanotechnology Materials and Devices Conference","volume":"41 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2010-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"12","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2010 IEEE Nanotechnology Materials and Devices Conference","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/NMDC.2010.5652331","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 12
Abstract
The detection of Hg2+ ions at the nanomolar level was achieved using a DNA aptamer as the molecular recognition element. The single stranded DNA sequence known as thrombin binding aptamer (TBA) was conjugated to a quantum dot and a gold nanoparticle to create the mercury-sensing probe. The quantum dot donor and gold nanoparticle acceptor allowed for optical detection via energy transfer using a spectrophotometer. The presence of the analyte caused a decrease in photoluminescence. The quenching efficiency at 500 nM Hg2+ ions with 10 nM aptamer probe was 62%. When the probe was exposed to a much higher concentration of Hg2+ ions, 824 µM, the photoluminescence was completely extinguished.