Allantoin as A Biomarker of Inflammation in an Inflammatory Bowel Disease Mouse Model: NMR Analysis of Urine

P. Dryland, D. Love, Michael F. Walker, Y. Dommels, C. Butts, D. Rowan, N. Roy, N. Helsby, B. Browning, Shuotun Zhu, B. Copp, L. Ferguson
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引用次数: 14

Abstract

Crohn's disease (CD) is an Inflammatory Bowel Disease (IBD) that is characterised by destructive inflamma- tion of the intestinal wall. Current methods for determining inflammation of the bowel are costly, time consuming and can cause discomfort to the patients. In order to address these problems, biomarker analysis of more accessible tissues is re- ceiving increasing attention. Oxidative stress has been implicated in the promotion of inflammation. Allantoin has re- cently been reported as a biomarker for oxidative stress in human serum and urine. This paper investigates allantoin as a biomarker of inflammation in a mouse model of CD. Proton nuclear magnetic resonance ( 1 H NMR) spectroscopy was used to analyse allantoin in urine from the mdr1a -/- mouse which is a model of CD. The data show that the levels of al- lantoin are strongly correlated with histological injury scores of mouse colonic tissue samples. Allantoin appears to be a useful biomarker of gut inflammation, involving oxidative stress, in a mouse model of CD and may be a potential bio- marker in human CD studies.
尿囊素作为炎症性肠病小鼠模型中炎症的生物标志物:尿液的核磁共振分析
克罗恩病(CD)是一种炎症性肠病(IBD),其特征是肠壁的破坏性炎症。目前确定肠道炎症的方法既昂贵又耗时,还会给患者带来不适。为了解决这些问题,生物标记物分析越来越受到人们的关注。氧化应激与炎症的促进有关。尿囊素最近被报道为人类血清和尿液中氧化应激的生物标志物。本文研究了尿囊素作为炎症的生物标志物在小鼠CD模型中的作用。利用质子核磁共振(1h NMR)光谱分析了CD模型小鼠mdr1a -/-尿液中的尿囊素。数据显示,尿囊素水平与小鼠结肠组织样本的组织学损伤评分密切相关。在小鼠乳糜泻模型中,尿囊素似乎是一种有用的肠道炎症(包括氧化应激)生物标志物,也可能是人类乳糜泻研究中潜在的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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