MOLECULAR-GENETIC CHARACTERISTICS OF MICROPARASITOCENOSIS MEMBERS IN OPISTORCHIASIS

Abstract

For the first time, an attempt was made to study the genome of Escherichia coli and Klebsiella pneumoniae strains isolated from patients diagnosed with opisthorchiasis. It was shown that 9 out of 13 investigated E. coli strains isolated from patients with opisthorchiasis invasion belonged to diarrheogenic groups: ETEC – O6, O8, O25; EPEC – O18, EIEC – O144; ExPEC – O1, O2. Whole genome sequencing of K. pneumoniae strains revealed that among 13 cultures isolated from patients with opisthorchiasis, 8 were assigned to one serotype O1 (v1, v2), which amounted to 53.8%. Serotype O5 accounted for 23.1% of strains, and isolated serotypes O2 and O3 were identified, which were detected in 80% of cases of infections caused by K. pneumoniae. Opisthorchiasis invasion to a greater extent affects the colonization of the human body by strains of E. coli and K. pneumoniae, carriers of clusters of pathogenicity and virulence genes, which is possibly associated with impaired immunity, or a more pronounced effect of Opisthorchis felineus metabolites. Key words: opisthorchiasis, microparasitocenosis, whole genome sequencing, Escherichia coli, Klebsiella pneumoniae