[Determination of secretory immunoglobulin A in urine of children with enzyme immunoassay].

Abstract

The estimation of secretory immunoglobulin A (sIgA) by an enzyme immunoassay (EIA) was used as heterogenic solid-phase assay. A monoclonal antibody directed against the human secretoric part and a peroxidase-marked anti-IgA serum may mediate the reaction. The test was evaluated on urine of 59 healthy children aged from 0-14 years. An age-dependent increase of sIgA concentration (mg/l) and excretion up to the 6th months of life was found. Thereafter the concentration did reach a constant value of 0.5 mg/l sIgA and 0.4 mg sIgA/g creatinine, respectively. The normal values may follow a logarithmic distribution. The presented method is highly sensitive, simple and safe.