EFFECT OF SOLVENT TYPE ON THE EXTRACTED BIOACTIVE COMPOUNDS OF MALLOW (MALVA SYLVESTRIS) LEAVE

Menoufia Journal of Food and Dairy Sciences Pub Date : 2018-06-01 DOI:10.21608/mjfds.2018.123879

M. El-sayed, M. A. Salem, I. Eisa

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Abstract

The effect of different extraction solvents (water, 70% ethanol and 95% methanol) on bioactive compounds (polyphenols, flavonoids and saponinand tanins) and antioxidant characteristics of mallow leaves extracts were investigated The total phenolic compounds extracted from green parts of mallow using 70% ethanol, 95% methanol and water were 57.2, 43.2 and 121.6 mg/g, respectively. The total flavonoids content of mallow water extract is significantly (p≤0.05) higher than those of methanolic and ethanolic extracts. Tannins content in mallow extract obtained by 70% ethanol was 15.52mg tannic/g which was significantly higher than the other solvents, meanwhile the lowest amount (4.9 mg tannic/g) was detected in 95% methanolic extract. The same trened was also found for saponin, since the highest value (6.7 mg/g) was in 70%ethanolic extract. Total antioxidant capacity (TAC) of mallow extracts at 500ppm decreased as the percentage of alcohol in the solvent increased. Whereas, the highest TAC (58.7±.1 %) was detected in water extract and decreased gradually by decreasing the percentage of water in the solvent. the water extract at 500ppm had significantly higher (p≤0.05) antioxidant activity compared to the other solvents. Consequently, the water was the best solvent for extraction phenolics and flavonoids from green parts of mallow and these extracts have a strong antioxidant activity

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溶剂类型对锦葵叶生物活性物质提取的影响

研究了不同提取溶剂(水、70%乙醇和95%甲醇)对锦葵叶提取物生物活性成分(多酚类、黄酮类、皂苷和单宁类)及抗氧化特性的影响。70%乙醇、95%甲醇和水提取锦葵绿部的总酚类化合物分别为57.2、43.2和121.6 mg/g。锦葵水提取物总黄酮含量显著(p≤0.05)高于甲醇和乙醇提取物。70%乙醇提取的锦葵提取物单宁含量为15.52mg /g，显著高于其他溶剂提取的锦葵提取物，95%甲醇提取的锦葵提取物单宁含量最低，为4.9 mg /g。同样的趋势也发现了皂苷，因为最高的值(6.7 mg/g)是在70酚提取物中。在500ppm浓度下，锦葵提取物的总抗氧化能力(TAC)随溶剂中酒精含量的增加而降低。而最高TAC(58.7±。在水提液中检测到1%)，并随着溶剂中水含量的降低而逐渐降低。500ppm水提物的抗氧化活性显著高于其他溶剂(p≤0.05)。结果表明，水是提取锦葵绿皮中酚类和黄酮类物质的最佳溶剂，其提取物具有较强的抗氧化活性

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Menoufia Journal of Food and Dairy Sciences

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