Imaging Cancer Cells Using a Holographic Microscope

Abstract

In this paper, a compact and high resolution in-line digital holographic microscope (DHM) is constructed that consisted of a 532 nm laser, mirrors, microscope objectives $(10\times,\boldsymbol{\text{NA}=0.25}$ and $\boldsymbol{40\times, \text{NA}=0.65}$) and CCD camera. Using the system, a series of holograms were successfully obtained and $\mathrm{n}\mu\text{merically}$ reconstructed to extract the three-dimensional measurements of phase, amplitude and the morphological structure of the tested cells. In this method, the quantitative biological measurements of different types cured cancer cells, involving A549, MCF-7 and SMMC-7721 cells, were all estimated to reveal biological structural information. Moreover, quantitative analysis of SMMC-7721 cell growth changes and drug response were discussed to supply significant information for the treatment of cancer cells.