ラットKupffer細胞と反応するモノクローナル抗体,UFT-4の作製とその免疫組織,電顕的解析

Abstract

A new monoclonal antibody, designated UFT-4, raised against rat Kupffer cells was produced using a sinusoidal liver cell-fraction as immunogen. In immunohistochemistry, UFT-4 reacted with Kupffer cells, interdigitating cells (IDC), sinus endothelial cells of the spleen, some reticulum cells, smooth muscle fibers and choroid plexus epithelia. Immunoelectron microscopy gave a positive reaction for a part of cytosol of Kupffer cells, IDCs, sinus endothelial cells of the spleen and some reticulum cells in a linear or filamentous fashion with some periodicity. On the other hand, blood monocytes and most macrophages in lymphatic sinus, lymphoid follicle, splenic red pulp and connective tissue were negative. The former cells, positive for UFT-4, almostly belong to a narrow spectrum of the classical reticuloendothelial system, explaining a close relationship between some endothelia and some macrophages or reticulum cells. The present result disclosed that UFT-4 is very usefull to study Kupffer cells and further to reestimate their origin and peculiar character in reticuloendothelial system.