Isolation and Characterization of Bacterial Protease Enzyme of Leather Waste

Abstract

The objectives of this study were to isolate and characterize bacteria which produced protease enzyme from tannery solid waste. A solid leather waste sample was used for bacterial isolation, taken from different waste warehouses (solid waste in unhairing phase). Several bacterial strains were isolated from the cultures in Petri dishes, after the growth of the colonies. These strains were characterized in terms of the production of proteolytic enzymes, by a method of screening on the media with casein, which allows the determination of proteolytic indices of microorganisms, the colony diameter, diameter of clear zone, proteolytic index, and enzymatic activities characterization on difference of pH and temperature.