Dongyang Hu, Jie Zhao, Jiancheng Wang, Shenghu Yan, Chengrui Wang, Qing Qing, Jiegao Zhu, Wenping Tao, Liqun Wang
{"title":"Pilot-scale Evaluation of an Anaerobic/Anoxic/Oxic Process for Nitrogen Removal from Sewage Using Metagenomic Sequencing","authors":"Dongyang Hu, Jie Zhao, Jiancheng Wang, Shenghu Yan, Chengrui Wang, Qing Qing, Jiegao Zhu, Wenping Tao, Liqun Wang","doi":"10.54097/ijbls.v2i3.8658","DOIUrl":null,"url":null,"abstract":"A modified pilot plant with two sequencing batch reactors on the strength of utilizing the inner carbon supply and adding suspended carriers was operated for 280 d to analyze nitrogen removal performance and microorganism community. Despite a low chemical oxygen demand (COD)/total nitrogen (TN) ratio of 3.5, the TN in the effluent decreased from 37.2 to 10.4 mg N/L. High-throughput sequencing indicated significant differences in the abundance of the phylum Actinobacteria (p<0.0001), Firmicutes (p<0.0001), Bacteroidetes (p<0.001), Uroviricota (p<0.0001) and Planctomycetes (p<0.0001) between the anoxic-carrier biofilms and flocculent sludge. Quantitative PCR revealed that denitrification and anammox genes were additional abundant in the anoxic-carrier biofilms than flocculent sludge (narG: p<0.0001; nirS: p<0.01; narH: p<0.01; norB: p<0.0001; hzsB: p<0.01; hdh: p<0.01). Thus, enrichment with denitrification and anammox bacteria might improve nitrogen removal; this was supported by KEGG pathway annotation. Collectively, this study suggests that anoxic-carrier biofilms might enhance nitrogen removal through partial denitrification and anammox process in CWWTPs.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"41 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biology and Life Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.54097/ijbls.v2i3.8658","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A modified pilot plant with two sequencing batch reactors on the strength of utilizing the inner carbon supply and adding suspended carriers was operated for 280 d to analyze nitrogen removal performance and microorganism community. Despite a low chemical oxygen demand (COD)/total nitrogen (TN) ratio of 3.5, the TN in the effluent decreased from 37.2 to 10.4 mg N/L. High-throughput sequencing indicated significant differences in the abundance of the phylum Actinobacteria (p<0.0001), Firmicutes (p<0.0001), Bacteroidetes (p<0.001), Uroviricota (p<0.0001) and Planctomycetes (p<0.0001) between the anoxic-carrier biofilms and flocculent sludge. Quantitative PCR revealed that denitrification and anammox genes were additional abundant in the anoxic-carrier biofilms than flocculent sludge (narG: p<0.0001; nirS: p<0.01; narH: p<0.01; norB: p<0.0001; hzsB: p<0.01; hdh: p<0.01). Thus, enrichment with denitrification and anammox bacteria might improve nitrogen removal; this was supported by KEGG pathway annotation. Collectively, this study suggests that anoxic-carrier biofilms might enhance nitrogen removal through partial denitrification and anammox process in CWWTPs.
在利用内供碳和添加悬浮载体的两个序批式反应器改造中试装置上运行了280 d,分析了脱氮性能和微生物群落。尽管化学需氧量(COD)/总氮(TN)比为3.5,但出水TN从37.2 mg N/L降至10.4 mg N/L。高通量测序结果显示,在缺氧载体生物膜和絮凝污泥中,放线菌门(p<0.0001)、厚壁菌门(p<0.0001)、拟杆菌门(p<0.001)、尿viricota (p<0.0001)和植物菌门(p<0.0001)的丰度存在显著差异。定量PCR结果显示,缺氧载体生物膜中反硝化和厌氧氨氧化基因比絮凝污泥中更丰富(p<0.0001;nirS: p < 0.01;narH: p < 0.01;norB: p < 0.0001;hzsB: p < 0.01;hdh: p < 0.01)。因此,反硝化和厌氧氨氧化菌的富集可能会提高氮的去除率;这得到了KEGG通路注释的支持。总之,本研究表明,厌氧载体生物膜可能通过部分反硝化和厌氧氨氧化过程增强CWWTPs中的氮去除。