Gold nano-particle based Al interdigitated electrode electrical biosensor for specific ssDNA target detection

R. Rajapaksha, U. Hashim, N. Natasha, M. Uda, V. Thivina, C. Fernando
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引用次数: 7

Abstract

Interdigitated electrode based electrical biosensors are prominent in biosensor field. The large number of finger electrodes as comb structure gain high sensitivity through electrical measurements. Listeria monocytogenes is a serious food borne pathogen based bacterium that can cause dangerous disease to human, some infection may result in death. In here, biosensor was prepared from 1μm gap Aluminum Interdigitated Electrode (Al IDE). Functionalization steps of the Al IDE to create biosensor was based on silanization by APTES, immobilization with Gold Nano particles (GNPs) mixed single stranded Listeria synthetic probe ssDNA with Au nano-particles and blocking with tween-20. Before and after functionalization the sensor was characterized morphologically using SEM images and structurally using EDX spectra. The functionalization steps were electrically characterized using current-voltage measurements. The selectivity of the biosensor with specific target was identified electrically using complementary, non-complementary and single base mismatch ssDNA targets. Blocking step with tween-20 was important to detect target specifically. The obtained variations in current indicate the varied concentrations of Listeria targets and it is confirmed that biosensor is suitable to detect different concentrations in the range from 10 fM to 10 μΜ.
基于金纳米颗粒的Al交叉指电极电生物传感器用于特异性ssDNA靶标检测
交叉指状电极型电生物传感器是生物传感器领域的热点。大量的手指电极作为梳状结构,通过电测量获得高灵敏度。单核细胞增生李斯特菌是一种严重的食源性致病菌,可对人类造成危险疾病,部分感染可致人死亡。本实验采用1μm间隙铝交叉指状电极(Al IDE)制备生物传感器。Al - IDE构建生物传感器的功能化步骤是基于APTES硅烷化、金纳米颗粒(GNPs)混合单链李斯特菌合成探针ssDNA与金纳米颗粒的固定化以及tween-20的阻断。功能化前后的传感器分别用SEM和EDX光谱进行了形貌表征和结构表征。利用电流-电压测量对功能化步骤进行了电表征。利用互补、非互补和单碱基错配的ssDNA靶标,电鉴定了具有特定靶标的生物传感器的选择性。20 -20阻断步骤对特异性检测目标具有重要意义。得到的电流变化表明李斯特菌靶标浓度的变化,证实了生物传感器适用于检测10 fM ~ 10 μΜ范围内不同浓度的李斯特菌。
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