Fabrikasi Ramah Lingkungan Komposit Nano Karbon Aktif-Partikel Perak dan Uji Aktifitas Antibakterinya

Abstract

Nanocomposite of activated carbon-silver nanoparticles (AC-AgNPs) have been successful greenly fabricated using ultrasonic (40 kHz; 2´50 watt) for various time of 10-, 20-, and 30-minutes agitation. The research was aiming to investigate the relationship of ultrasonic agitation times and antibacterial activity of the associated nanocomposite mentioned. For that, the first step performed was synthesize silver nanoparticles (AgNPs) from silver nitrate salt (AgNO3) (10 ml,3 mM) using aqueous-pineapple extract of various concentration of approximately 10, 20, 30, 40 and 50 % w/v. The second step was fabricated activated carbon nanoparticles (ACNPs) from local commercially available carbon black fine powder through the advanced top-down method. Finally, properly mixed the produced ACNPs and AgNPs under ultrasonic agitation as mentioned above. Analysis and characterization of the desired produced materials were conducted by using Ultra violet-visible (UV-Vis) spectroscopy, Transmission electron microscopy (TEM), and Fourier transformed infrared (FTIR) spectroscopy. It was well known that about 10-50 nm in size of AgNPs were successful produced with spherical particles in shape. The AgNPs could then be incorporated and spread out evenly on the surface of the ACNPs matrix. In this situation, FTIR spectra confirmed that the AgNPs mentioned particularly interact with each other with hydroxyl (-OH) and carbonyl (C=O) groups existing on the surface of the ACNPs matrix. The antibacterial activity of the produced AC-AgNPs nanocomposite was separately tested toward both E. coli and S. aureus bacterial using agarose-filter paper disc method. It was known that the clear zone generated around the disc was about 1.23 mm and 2.07 mm in averages for Eschericia coli (E. coli) and Staphylococus aureus (S. aureus) respectively.