Non-specific resistance of the rabbits organism affected by causative pathogen Treponema cuniculi and Eimeria sp.

Abstract

One of the actual rabbits breeding problem is the reduction of their resistance, which is caused by the spread of invasive diseases, especially associative, which is caused by the Treponema cuniculi and Eimeria sp. The aim of the work was to establish the effect of spirochetosis and eimeriosis on the indicators of non-specific resistance of the rabbits organism. The study was conducted on 59 male rabbits aged 3-5 months of the Californian breed, selected by analogy. Animals were divided into two groups: healthy animals (control group) and sick animals (research group). Intensity of invasion was determined by Mac-Master method. It has been established that the level of rabbits invasion by spirochetosis and eimeriosis was, on average, 1155.17±184.87 and 6668.97±284.16 pathogens in 1 g of feces. The definition of phagocytic activity of neutrophils was carried out with the addition of standardized to 2000000000/ml suspension of daily culture of E. coli 055K59№3912/41. The bactericidal activity of blood serum was determined by Smirnova A.V. and Kuzmina T.A. method with E. coli microbial test-culture 055K59№3912/41. The lysozyme activity in blood serum was determined by Nephelometric method using the Dorofachuk V. G. method with microbial test-culture Micrococcus luteus ATSS9341. Circulating immune complexes were determined by using polyethylene glycol in borate buffer. It was found that phagocytic activity in blood of sick animals is lower than in blood of healthy ones by 5.17% (p<0.05). A low indicator of phagocytic activity shows depressed phagocytosis in the organism of animals suffering from spirochetosis and eimeriosis of rabbits. The phagocytic number in the blood of rabbits of the experimental group was significantly lower by 9.70% (p<0.05), as compared to the control group and correlated with the index of phagocytic activity. The important elements of immunity are indicators of bactericidal and lysozyme activity of blood serum. Low bactericidal activity of serum by 19.95% (p<0.001) and lysozyme activity by 8.34% (p<0.001) in rabbits organism affected by causative pathogens (Treponema cuniculi and Eimeria sp.) also indicates a weakening of the factors of non-specific natural resistance of the organism. Analyzing the level of circulating immune complexes, it was found a high levels of medium (8,28±0,53 vs 5,93±0,41) and small CIC (8,25±0,60 vs 4,93±0,59) for associative disease, respectively,  by 1.40 (p<0.001) and 1.67 times (p<0.001) against the control. It indicates the inhibition of the immunobiological activity in the organism of rabbits as a result of the compound of specific antibodies with the products of the exchange of helminths.