Xylanase and Its Industrial Applications

A. Basit, Wei Jiang, Kashif Rahim
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引用次数: 9

Abstract

Lignocellulosic biomass is a renewable raw material. Industrial interest with new technology has grown to take advantage of this raw material. Different microbial enzymes are treated with biomass to produce the desired products under ideal industrial conditions. Xylanases are the key enzymes that degrade the xylosidic linkages in the xylan backbone of the biomass, and commercial enzymes are categorized into different glycoside hydrolase families. Thermophilic microorganisms are an excellent source of thermostable enzymes that can tolerate the extreme conditions of industrial processing. Thermostability of xylanases from thermophilic microorganisms has given the importance for a specific activity at elevated temperatures and distinction due to biochemical properties, structure, and mode of action. Optimized xylanases can be produced through genetic engineering: a novel xylanase is isolated from an extreme environment and then genetically modified to improve suitability for industrial contexts. Recombinant protein techniques have made it possible to engineer and express thermostable xylanases in bacteria, yeasts, and filamentous fungi. We will discuss the biotechnological potential of xylanases from thermophilic microorganism and the ways they are being optimized and expressed for industrial applications.
木聚糖酶及其工业应用
木质纤维素生物质是一种可再生原料。工业对利用这种原料的新技术的兴趣日益浓厚。在理想的工业条件下,用生物质处理不同的微生物酶以生产所需的产品。木聚糖酶是降解生物质木聚糖主链中木苷键的关键酶,商业酶分为不同的糖苷水解酶家族。嗜热微生物是耐热酶的极好来源,可以忍受工业加工的极端条件。来自嗜热微生物的木聚糖酶的热稳定性对其在高温下的特定活性和生物化学性质、结构和作用方式的区别具有重要意义。优化的木聚糖酶可以通过基因工程生产:从极端环境中分离出一种新的木聚糖酶,然后对其进行基因改造,以提高其对工业环境的适应性。重组蛋白技术使得在细菌、酵母和丝状真菌中设计和表达耐热木聚糖酶成为可能。我们将讨论来自嗜热微生物的木聚糖酶的生物技术潜力,以及它们在工业应用中的优化和表达方式。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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