Two-in-one-interferometer OCT system for bioimaging

Abstract

rectly with histological sections with use of markers to correlate position. For 100 microwatt powers at the surface, we were to obtain image information at depths 2.5 mm, enabling imaging through the entire rat cortex. Figure 1 shows a lateral-medial B-scan of a healthy rat neocortex at the midpoint taken with use of 1300 nm light. The normal cortex (here approximately 2.1 mm in thickness) is a sklayered structure defined by the morphology and density of the neurons in each layer. While we are not able to differentiate specific layers in the cortex, the cortexwhite matter boundary is clearly visible. Figure 2 displays the lateral-medial B-scan in the parietal region from a rat with cortical dysplasia. Cortical dysplasia is manifested by a thinning of the cortex, to 0.5 mm, and a loss of differentiation between the cortex layers. The hippocampus, heterotopic neurons, and the boundary between hippocampus and ventricle are also visible in Fig. 2. Additional structures have also been detected in OCT scanning, including the dura matter, blood vessels, and artificially produced hemorrhages. Subsequent histological analysis shows a good correlation with the OCT tomograms, particularly within the cortical surface layers and the boundary between gray and white matter. *Institute of Applied Physics of the Russian Academy of Sciences, 603600 Nizhny Novgorod, Russia **Department of Physics and Brain Institute, University of Florida, Gainesville, Florida 3261 1 1. J. A. Izatt, M. R. Hee, E. A. Swanson, C. P. Lin, D. Huang, J. S. Schuman, C. A. PuTw~color-in-oneinterferometer OCT system for bioimaging