Functionalised polyaniline nanowires: A prospective biosensing platform

Abstract

In this work we have used functionalized polyaniline (PAn) nanowires for fabrication of a biosensing platform which was exploited for fabrication of three different biosensors. In one effort, we have immobilized single-stranded DNA oligonucletide (ssdA) on this platform and have monitored its combination with the complementary strand (ssdT) at a concentration as low as 10-18 M. Moreover we have immobilized glucose oxidase on this platform that could be used for amperometric detection of glucose in aqueous solution over a wide concentration range of 1μM-20mM. We could successfully immobilize Lamin A (LA) antibody on the platform and its response towards the Lamin A protein could be nicely demonstrated down to a concentration as low as 10 μm. Therefore, the efficiency of the general biosensing platform is established.