In Vitro Effects of Brassinosteroids on the Growth and Antioxidant Enzyme Activities in Groundnut

ISRN Agronomy Pub Date : 1900-01-01 DOI:10.5402/2012/356485

A. Verma, C. Malik, V. Gupta

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引用次数: 36

Abstract

This paper deals with the in vitro effects of brassinosteroids (BRs) on growth in the form of multiple shoots, chlorophyll content, Hill reaction activity (HRA), activities of catalase (CAT), peroxidase (POX), polyphenol oxidase (PPX), and ascorbate peroxidase (APX) in Arachis hypogaea L. genotypes (M-13 and PBS24030). In vitro impact of BR on shoot multiplication potential was found to be the best at 1 mL L−1 with BA (3 mg L−1) in both the cultivars. Flowering was observed in PBS24030 on the medium containing 2.0 mL L−1 BR with 3 mg L−1 BA. Rhizogenesis was noticed in the presence of BR alone. Total chlorophyll content and HRA were highest at 2.0 mL L−1 with BA in M-13 and 1.0 mL L−1 with BA in PBS24030. Antioxidant enzyme activities were increased in the presence of BR whether alone or in combination with BA in both the cultivars. However, progressive decline was observed in case of MDA content. The results obtained in the study clearly indicated not only the in vitro establishment of groundnut cultivars in the presence of BR alone and in combination with BA but also its effect on various growth promotory physiological parameters.

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油菜素内酯对花生生长和抗氧化酶活性的影响

研究了油菜素内酯(BRs)对花生多芽生长、叶绿素含量、Hill反应活性(HRA)、过氧化氢酶(CAT)、过氧化物酶(POX)、多酚氧化酶(PPX)和抗坏血酸过氧化物酶(APX)活性的体外影响。在1 mL L−1添加BA (3 mg L−1)时，BR对芽部增殖潜力的影响最大。PBS24030在含有2.0 mL L−1 BR和3 mg L−1 BA的培养基上开花。BR单独存在时，可以观察到根的发生。总叶绿素含量和HRA最高，M-13和PBS24030分别为2.0 mL L−1和1.0 mL L−1。无论是单独添加BR还是与BA联合添加，两个品种的抗氧化酶活性都有所提高。然而，在MDA含量逐渐下降的情况下观察到。本研究结果不仅明确了BR单独存在和BA联合存在对花生品种体外培养的影响，而且明确了BR对花生各种促生长生理参数的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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ISRN Agronomy

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