Construction of Aseptic Culture System for Spores of Sphaeropteris lepifera

Abstract

Through the aseptic culture test of Sphaeropteris lepifera (J Sm. ex Hook.) R. M. Tryon, try to screen out the best conditions for each process of aseptic culture for spores of Sphaeropteris lepifera, in order to establish a systematic, efficient and complete aseptic culture technology system for spores of Sphaeropteris lepifera, and try to achieve factory production of Sphaeropteris lepifera seedlings. The mature dark brown spores off leaf from Sphaeropteris lepifera were used as explants, disinfected with 75% alcohol for 10 s and 0.1% Hg for 6 min. The induced germination medium is 1/5 MS+6-BA 0.5 mg/L+IBA 0.5 mg/L. The proliferation medium is 1/2 MS + 6-BA 0.3 mg/L+ IBA 0.05 mg/L. The differentiation medium is MS+6-BA 0.05 mg/L+ IBA 0.05 mg/L. And the rooting medium is MS + 6-BA 0.2 mg/L + IBA 0.2 mg/L. The culture temperature is (25±2)°C, the light source is fluorescent, the illumination time is 12 h/d, and the illumination intensity is 2000 lx.