Manipulating the Assembly of DNA Nanostructures and Their Enzymatic Properties by Incorporating a 5′-5′ Polarity of Inversion Site in the G-Tract

IF 5.1 Q1 POLYMER SCIENCE
Yanwei Cao, Wenjing Li, Renjun Pei*
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引用次数: 1

Abstract

Supramolecular DNA complexes consisting of both DNA duplexes and tetrameric G-quadruplexes are fabricated successfully by utilizing a single short DNA strand that contains one 5′-5′ polarity of inversion site in the middle of G-tract. The resulting DNA supramolecules exhibit significantly high peroxidase activities after interaction with hemin due to the presence of various G-quadruplex–duplex (G4–duplex) interfaces. Significantly, we find that the addition of a C-rich fragment to the designed sequence not only allows the self-assembly of two-dimensional porous DNA nanostructures via the formation of dimeric i-motif structures but also could act as a control element to facilitate the generation of pH-sensitive G4-based DNAzymes. The enhanced catalytic activity obtained from specific sequence modifications as well as the controllable feature of these DNA nanostructures can significantly benefit further applications of DNA functional materials in complex biological systems.

Abstract Image

通过在g通道中加入5 ' -5 '极性反转位点来操纵DNA纳米结构的组装及其酶性质
利用一条短的DNA链,在g束中间含有一个5 ' -5 '极性的反转位点,成功地制备了由DNA双链和四聚体g -四聚体组成的超分子DNA复合物。由于多种g -四双工(g4 -双工)界面的存在,产生的DNA超分子在与血红蛋白相互作用后表现出显著的高过氧化物酶活性。值得注意的是,我们发现在设计的序列中添加一个富含c的片段不仅可以通过形成二聚体i-motif结构来实现二维多孔DNA纳米结构的自组装,而且还可以作为一个控制元件来促进ph敏感的g4基DNAzymes的产生。这些DNA纳米结构通过特定的序列修饰获得的催化活性增强,以及这些DNA纳米结构的可控特性,将极大地促进DNA功能材料在复杂生物系统中的进一步应用。
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来源期刊
CiteScore
10.40
自引率
3.40%
发文量
209
审稿时长
1 months
期刊介绍: ACS Macro Letters publishes research in all areas of contemporary soft matter science in which macromolecules play a key role, including nanotechnology, self-assembly, supramolecular chemistry, biomaterials, energy generation and storage, and renewable/sustainable materials. Submissions to ACS Macro Letters should justify clearly the rapid disclosure of the key elements of the study. The scope of the journal includes high-impact research of broad interest in all areas of polymer science and engineering, including cross-disciplinary research that interfaces with polymer science. With the launch of ACS Macro Letters, all Communications that were formerly published in Macromolecules and Biomacromolecules will be published as Letters in ACS Macro Letters.
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