Electrical Impedance Spectroscopy Microflow Cytometer for Cell Viability Tests

Abstract

The micro flow cytometer presented here is a combination of a set of coplanar electrodes and a microfluidic channel to test cell viability based on Single-cell Electrical Impedance Spectroscopy analysis. The device was tested both with a control and with a treated HeLa cells suspension. Impedance signals acquired were filtered and processed to obtain the data, classifying and clustering the results by dead and living cells to make the cell viability test. Results were compared with the standards of MTT and Neubauer Chamber viability tests, showing that 0.83μL of sample in the prototype designed and fabricated in this work was enough to obtain an approximate response of them.