"Facile Synthesis of Chitosan Nanoparticle with Different Features and Histopathological Ultrastructural Study on The Effect of Its Bio-complex with Bovine Serum Albumin in Mice Liver"

Abstract

Synthesis with Different Features and Histopathological Ultrastructural Study on The Effect of Its Bio-complex with Bovine Serum Albumin in Mice We have synthesized chitosan nanoparticles (CS-NPs) with different characteristics using ionic gelation method. Results showed that the synthesized CS-NPs with different concentrations of trisodium polyphosphate (TPP) are irregular - spherical shaped with narrow size distribution and high stability. The microporous chitosan was also synthesized with new features characterized with different techniques as SEM, EDX, FTIR and BET. The synthesized microporous chitosan has a surface area and pore volume of 245.571m 2 /g and 0.155cm 3 /g respectively. Its microporous surface is functionalized with carboxyl and amine groups. Histopathological and ultrastructural studies of mice liver were performed using optical and scanning electron microscopy (SEM) by intraperitoneal (IP) injection of male mice to evaluate toxicity of free and BSA-CS-NPs inside the body after three, six and nine days. However, no pathological or illness signs were observed in mice throughout the in vivo experiment with non-significant changes on body and relative weight of liver in compared to the negative control group. In addition, biochemical parameters exhibited a normal functionality of liver after BSA-CS-NPs administration. Accordingly, mild histopathological or ultrastructural alterations were observed in the liver of treated mice in all groups with respect with the negative controls. Activated Kupffer cells and leukocytes were observed due to the synergism of adjuvant effect of CS-NPs and antigenic peptides involved in BSA. Herein, we opened new direction for synthesis of different forms of CS-NPs and providing an obvious insight on histopathological and ultrastructural changes after administration of CS-NPs and its protein bio-complexes.