A Bioethanol Waste Challenge being a Source of High-Quality ProteinRich Feedstock: A New Technology

OA Almazan-del Olmo Biotechnology Division
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Abstract

Microbial biomass protein from Candida utilis yeast was produced using distillery vinasse as the sole source of carbon and energy. The process was carried out from lab scale to 200 m3 fermentors. Propagation media have to be supplemented with nutrients since vinasse cannot provide all compounds needed for a stable operation. Different mixtures of molassesenriched vinasse were tested and finally, a Microbial Growth Enhancer (MGE) was incorporated into the media to ensure its optimization. MGE-supplemented vinasse at 0.03 kgm3 was found optimum for yeast propagation. At an industrial scale, µmax ≥ 0.33 h-1 was obtained which allowed a stable behavior of the culture at this scale and significant industrial productivity. Yeast biomass from vinasse showed a composition and amino acid profile very similar to those previously obtained from molasses medium. Nutritional tetestsemonstrated that vinasse´s yeast is an excellent source of proteins for animal feeding and an efficient pollution reducer.
将生物乙醇废料转化为优质蛋白质原料的挑战:一项新技术
利用酒厂沼渣作为唯一的碳和能量来源,从利用念珠菌酵母中生产出微生物生物质蛋白。该过程的规模从实验室到 200 立方米的发酵罐。由于酒糟不能提供稳定运行所需的所有化合物,因此必须在繁殖培养基中添加营养物质。对不同的富含糖蜜的蔗渣混合物进行了测试,最后在培养基中加入了微生物生长促进剂(MGE),以确保其最优化。发现添加了 0.03 kgm3 MGE 的蔗渣最适合酵母繁殖。在工业规模上,µmax ≥ 0.33 h-1,这使得培养物在这一规模上表现稳定,并具有显著的工业生产力。从蔗渣中提取的酵母生物质显示出与以前从糖蜜培养基中提取的酵母生物质非常相似的成分和氨基酸谱。营养测试表明,蔗渣酵母是动物饲料蛋白质的极佳来源,也是一种有效的污染减少剂。
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