"Replacement of Animals Used in the Maintenance of Aedes Aegypti by Alternative Methods. Refinement of Procedures That Minimize the Discomfort of Insect Maintenance by Swiss Webster Mice"

Abstract

One of the most critical points in Laboratory Animal Science (CAL) is the use of some species as a blood supplier for food (Blood Meal) and breeding of hematophagous insects, vectors of serious and (often) neglected diseases. In summary, the main objectives of the project are: Aedes , we will test the application of two refinements in the procedure: The first is based on the use of Erythropoietin, increasing the number of and we hope to speed up the time to food satiety for the mosquito and the second to use an analgesic, an opiate with minimal adverse effects. Complementing, in the case of successful use of Erythropoietin. We will apply the association of the recombinant hormone with the analgesic for mice during the Aedes aegypti Blood Meal in the laboratory aiming, firstly, to avoid the use of the animal. However, if it is not possible to apply it in the maintenance routine of Aedes aegypti to minimize the stress and discomfort of this animal during feeding and creation of hematophagous vectors, in our case, the mosquito.