The Effect of Monomycin and Gentamycin Sulfate on Growth of Promastigotes of Leishmania Under In Vitro Conditions

Y. Garedaghi, Y. Firouzivand, Behrad Khan Ahmadi, Alireza Zarei, Ebrahim Salehizadeh
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Abstract

Introduction: Given the prevalence of leishmaniasis in some parts of Iran and the medical problems caused by it and considering some reports on the effectiveness of monomycin in treating the disease, this study was performed to determine the effect of this drug on Leishmania major promastigotes in vitro. Materials and Methods: The study was conducted experimentally. Drug films containing 15% monomycin sulfate and 0.5% gentamicin sulfate were prepared using ethyl cellulose and hydroxypropyl methyl cellulose (HPMC) with the aim of developing a treatment for cutaneous leishmaniasis. In order to investigate the mechanism of drug release and its effect on parasites, the modified Novy-MacNeal-Nicolle (NNN) medium was used for the amplification of the parasites and the growth inhibition zone around the drug release areas was measured. The studied environments included environments containing drug discs, environments without drug discs, and environments with placebo discs. The rates of dead and live parasites were determined in these environments and statistically compared using chi-square test. Results: It was revealed that the growth of parasite colonies was inhibited in the plates containing drug films. In 3-cm halo around the drug film, no parasite growth was observed and promastigotes were observed to be dead and degenerated. However, in the plates containing placebo and medium without film, parasitic colonies were observed. The survival rate of the parasites in placebo and drug-free plates was 96% and it was 7% in the drug plate (P<0.000). Conclusion: Monomycin and gentamicin sulfate drug discs have good potential for gradual release. They affect the growth of the parasite and can be used in humans.
单霉素和硫酸庆大霉素对利什曼原虫原鞭毛菌体外生长的影响
导论:鉴于利什曼病在伊朗部分地区的流行及其引起的医疗问题,并考虑到一些关于单霉素治疗该病有效性的报道,本研究旨在确定该药物对体外利什曼原虫原鞭毛虫的影响。材料与方法:采用实验方法。采用乙基纤维素和羟丙基甲基纤维素(HPMC)制备了含有15%硫酸单霉素和0.5%硫酸庆大霉素的药膜,目的是开发一种治疗皮肤利什曼病的药物。为了研究药物的释放机制及其对寄生虫的影响,采用改良的NNN (Novy-MacNeal-Nicolle)培养基对寄生虫进行扩增,并测定了药物释放区周围的生长抑制带。研究的环境包括有药物盘的环境、没有药物盘的环境和有安慰剂盘的环境。测定这些环境中寄生虫的死、活率,并用卡方检验进行统计学比较。结果:药膜板能抑制寄生虫菌落的生长。药膜周围3cm光晕内未见寄生菌生长,原毛体已死亡退化。然而,在含有安慰剂和不含膜的培养基中,观察到寄生菌落。在安慰剂和无药培养皿中,寄生虫的存活率为96%,在有药培养皿中为7% (P<0.000)。结论:单霉素和硫酸庆大霉素药盘具有良好的缓释潜力。它们会影响寄生虫的生长,并可用于人类。
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