Hematological Changes in Diabetic Rats Receiving Melatonin, Vitamin D and Vitamin E are Not Reliable Indices of Inflammatory Changes

Abstract

Background: Finding a suitable model to study the effect of various treatments on diabetes will help to avoid undesirable effect on humans during empirical investigation. This study aims to evaluate the values of biomarkers such as Neutrophil-Lymphocyte Ratio (NLR), Platelet-Lymphocyte Ratio (PLR), and Lymphocyte-Monocyte Ratio (LMR) in monitoring diabetes in a rat model subject to treatment with melatonin, vitamin-D and vitamin-E. Methods: Male albino rats (n=8-10 per group), normal and with diabetes were divided as follows into 12 groups: G1 normal fed, received no medications; G2 normal, treated with melatonin; G3 normal, treated with vitamin-E; G4 normal, treated with vitamin-D; G5 diabetic; received no medications; G6 diabetic, treated with insulin; G7 diabetic treated with melatonin; G8 diabetic, treated with melatonin and insulin; G9 diabetic, treated with vitamin-E; G10 diabetic, treated with vitamin-E and insulin; G11 diabetic, treated with vitamin-D and G12 diabetic, treated with vitamin-D and insulin. Two months post-treatment, hematological (NLR, PLR and LMR) and biochemical examination of glucose profile and oxidative stress status, were performed. Results: NLR is significantly decreased on comparing G3 and G4 with G1, and significantly increased on comparing G9 with G1. On comparing G3 with G5 and G6, NLR is significantly decreased, but on comparing G9 with G5 and G6, NLR is significantly increased. However, PLR and LMR showed no statistically significant changes in all rat groups. Conclusion: Hematological changes in diabetic rat model receiving melatonin, vitamin D and E are not reliable indices of inflammatory changes.