In-vitro sub-cellular sampling of metabolites in biological samples

Deepa Raghu, Q. Xia, Linwen Zhang, A. Vertes, M. Reeves
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Abstract

Chemical analysis of metabolites directly from cell samples at sub-cellular level in ambient conditions is essential to understand the biochemical pathways in these organisms. In order to achieve this goal we combine near-field ablation with mass spectrometry. As a first step towards the integration of near-field ablation with mass spectrometry, we will report preliminary results of the chemical analysis of Arabidopsis Thaliana.
生物样品中代谢物的体外亚细胞取样
在环境条件下,直接从细胞样本中提取代谢物进行亚细胞水平的化学分析对于了解这些生物的生化途径至关重要。为了实现这一目标,我们将近场烧蚀与质谱相结合。作为近场烧蚀与质谱结合的第一步,我们将报道拟南芥化学分析的初步结果。
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