Species Recognition of Aspergillus Conidia Using Convolutional Neural Networks in Scanning Electron Microscopy Imagery

Huaizhong Zhang, Marta Filipa Simões
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Abstract

— This paper presents a practical recognition method based on deep learning techniques, for fungal species, through Scanning Electron Microscopy (SEM) images. A small number of images was acquired. To circumvent the issue of not having many samples, a method of generating the training set is proposed to increase target signatures and optimize the baseline quality of inputs for object recognition. To tackle the challenge of detecting varied scale targets, a sophisticated and powerful Convolutional Neural Network (CNN) based on faster region R-CNN, with the prepared training dataset, was trained. In this study, the datasets for five different species of Aspergillus were previously collected via SEM. The proposed method is applied to identify the spore structures – conidia – in the images so as to recognize the species respectively. The initial experimental results show that the developed method can qualitatively and quantitatively identify the relevant species effectively, being of major importance for the development of easier diagnostic and identification tools in mycology.
扫描电镜图像中基于卷积神经网络的分生曲霉物种识别
-本文提出了一种基于深度学习技术的实用识别方法,通过扫描电子显微镜(SEM)图像识别真菌物种。获得少量图像。为了避免样本数量不足的问题,提出了一种生成训练集的方法,以增加目标签名并优化目标识别输入的基线质量。为了解决检测不同尺度目标的挑战,使用准备好的训练数据集,基于更快的R-CNN区域训练了一个复杂而强大的卷积神经网络(CNN)。在本研究中,通过扫描电镜收集了五种不同曲霉的数据集。将该方法应用于识别图像中的孢子结构分生孢子,从而分别识别物种。初步实验结果表明,所建立的方法能够有效地对相关菌种进行定性和定量鉴定,对于开发更简便的真菌学诊断和鉴定工具具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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