Cryo-electron microscopy of vitrified specimens: an approach to the study of bulk specimens.

I Erk, H Delacroix, G Nicolas, J L Ranck, J Lepault
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引用次数: 2

Abstract

We are using and developing cryo-electron microscopy of vitrified specimens. Our main interests concern the structure of muscle and muscular components. Micrographs which generally contain periodic features are analyzed by numerical image processing methods. To detect artifacts induced by the electron microscopy techniques, we correlate our results to those obtained by X-ray diffraction. In this paper, we describe our approach to the study of bulk specimens. Vitrification of such specimens is assessed by cryo-X-ray diffraction. Microscopy is done on cryo-substituted specimens.

玻璃化标本的低温电子显微镜:研究大块标本的一种方法。
我们正在使用和发展冷冻电子显微镜的玻璃化标本。我们的主要兴趣在于肌肉的结构和肌肉成分。显微照片通常包含周期性特征,用数值图像处理方法对其进行分析。为了检测由电子显微镜技术引起的伪影,我们将我们的结果与x射线衍射得到的结果联系起来。在本文中,我们描述了我们的方法来研究散装标本。这种标本的玻璃化是通过冷冻x射线衍射来评估的。显微镜是在冷冻代替的标本上进行的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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