Indirect regeneration of local potato varieties

Abstract

The study considers three local strains of potato such as Aksor, Tokhtar, Astanalyk. Strains were investigated on the tendency of callusogenesis and ability of indirect in vitro regeneration. As a result of the investigation, there was studied the function of phytohormones. The aim of the experiment was to study the dependence of the callus formation and indirect regeneration of potato strains from the growth regulators. It was identified that the intensity of the callus formation from the stem explants were effective for the Aksor (93%) and Tokhtar (87%) strains in MSK II growth medium, containing 2,0 mg/l BAP and 3,0 mg/l NAA, and variation MSK V, containing 4,0 mg/l BAP and 1,0 mg/l NAA, were optimal for the Astanalyk (82.5%) strain. Frequency of regeneration in MSR III growth medium, containing 0.5mg/l BAP, trans-zeatin 1.0 mg/l, GA3 2.0 mg/l, was 53,3% and 77,8% in Aksor and Tokhtar, respectively. Astanalyk strain showed 60,9% regeneration in MSR IV growth medium, containing IAA 0,1 mg/l, trans-zeatin 1.0 mg/l, GA3 10.0 mg/l. The study concluded that local potato strains have a high ability for callus formation and indirect regeneration.