Advantages Of Application Of Algorizobacterial Consortia In Cultivation Of Legume Crops

S. Didovich, І. А. Maltseva
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Abstract

Possibility of creation of effective algobacterial consortia with high nodulе ability of rhizobial cells is experimentally proven. Pre-sowing bakterization treatment of chickpea seeds provided the increase of the productivity of plants such polyfunctional cyanorhizobial complex by 43,4% in the conditions of the field experiment. It may serve as a base for biological technology of growing chickpea for obtaining of ecologically safe production. We brought under cultivation 12 strains associated with a strain of cyanobacteria Nostoc linckia 144, identified by their morphological, cultural, physiological, and biochemical properties. We revealed that on nitrogen-free substrate some 62 and 77% of strains satellite cyanobacteria and algae itself positively affect tuberization of soybeans and chickpeas and increase the dry weight of aboveground plant up to 28,6-35,7%; under condition of Southern black soils the bacterization of strains of Microbacterium laevaniformans KAGK and Mesorhizobium siceri raised the ground dry weight of plants per 0.82 g / plant (21.1%) compared with mono-farmery. We created the effective algological bacterial consortium of high activity of Mesorhizobium siceri cells, whose number was 9.7 h105 BUOD / mL, which exceed by two orders of magnitude higher the monoculture rhizobia. We proved experimentally the efficiency of usage of cyanobacteria combined with nodule bacteria in agrobiotechnology and suggested the high efficiency of prebacteryzation of seed by cyan-rhyzo-consortium of chickpeas which ensuring the increase of plant productivity by 43.4%.
藻歧菌群在豆科作物栽培中的应用优势
实验证明了产生具有高nodulе根瘤菌细胞能力的有效藻菌联合体的可能性。在田间试验条件下,对鹰嘴豆种子进行播前发酵处理,可使该多功能氰根瘤菌复合体的产量提高43.4%。可作为鹰嘴豆生物栽培技术的基础,实现鹰嘴豆的生态安全生产。我们培养了12株与蓝藻Nostoc linickia 144菌株相关的菌株,通过其形态,培养,生理和生化特性进行了鉴定。结果表明,在无氮基质上,62%和77%的卫星蓝藻菌株和藻类本身对大豆和鹰嘴豆的结核产生积极影响,使地上植物的干重增加28.6 ~ 35.7%;在南方黑土条件下,枯草微杆菌KAGK和西西里中根瘤菌的抑菌作用比单作提高植株地面干重0.82 g /株(21.1%)。我们培育出了高效菌群,其数量为9.7 h105 BUOD / mL,比单作根瘤菌高出2个数量级。通过实验验证了蓝藻与根瘤菌结合在农业生物技术中的应用效率,并提出了鹰嘴豆蓝藻与根瘤菌结合的种子预杀菌效率高,可使植物产量提高43.4%。
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