Antibiotic Resistance Gene Expression of Multi-Drug Resistant Gram-Negative Bacteria Isolated in a Military Hospital of Vietnam

Abstract

Hospital-acquired infection is one of the main causes for prolong treatment and risk of death. In this study, nine multidrug resistant strains were isolated from patient’s samples. The antimicrobial susceptibility testing was performed to confirm the strong resistance of nine strains with β-lactam, carbapenem and colistin. The expression of resistance genes was examined using reverse transcription PCR to identify 14 genes associated with drug resistance and the genotype matched the phenotype. Two carbapenem resistance genes (blaNDM-1 and blaKPC-2) and one colistin resistance gene (mcr-1.1) were found in this study, indicating a likely shared origin of these resistance genes and the need of understanding the processes of drug resistance development, transmission and spread.