STUDY OF ACUTE TOXICITY AND ANTITUMOR ACTIVITY OF NEW COLCHAMETIN PREPARATION СOLCHAMETIN

Abstract

The aim of the study was to study acute toxicity in different methods of administration in mice and rats of a new antitumor preparation "colchametin" (K-2) and its antitumor activity on 2 strains of tumors Material and research methods. Acute toxicity of the preparation K-2 with a single intraperitoneal administration was carried out according to the Litchfield and Wilcoxon method in 60 white infertile mice weighing 202g. both sexes and 60 male and female rats weighing 14010g 5 animals in each group, a total of 120 mice and rats were used. The study of antithumor activity was performed on 12 non-fertile and 16 linear mice with transfused tumors S-180 and ACATON, which was injected with preparations on the 4th day after tumour was administered on day 4 after tumour transfusion 10 times. Evaluation of the results was carried out according to standard criteria: inhibition of tumor growth (SRW), body weight and spleen of animals. Differences at p < 0.05 were considered reliable. Results. Such data at single vnutribryushinny introduction are obtained to mice: the average lethal dose of LD50 makes 890 (-150+172) mg/kg, At oral introduction of medicine K-2 to mice of LD50 is equal to 3250 (-630+650) mg/kg, LD50 at single vnutribryushinny to rats of medicine K-2 LD50 makes 410 (-56+61) mg/kg, LD50 at single oral introduction to rats makes 3250 (-630+650) mg/kg, are defined also at all ways of introduction of LD10, LD16 and LD84 value The antitumor activity of the drug K-2 on the tumor strain of Sarcoma 180 was high - about 99/90%. On the ACATON tumor, the K-2 effect during intraperitoneal administration was lower and reached 76/75%, however, its effect on both tumors was accompanied by a decrease in hematopoietic indicators. Conclusions. The study of the acute toxicity of the substance of the preparation K-2 showed that this preparation belongs to class IV of low-toxic compounds. On 2 tumors, the effect of the new drug was high, but hematopoietic indicators were reduced.