Preparation and characteristics of diatrizoate meglumine-loaded chitosan microspheres

Abstract

The purpose of the present study is to prepare a diatrizoate meglumine-loaded chitosan microsphere, which could be used to be investigated by ultrasonic imaging technique in vivo and would has water absorption swelling property to dredge the obstruction. Diatrizoate meglumine-loaded chitosan microspheres were prepared by crosslink emulsification process, to use chitosan acetic acid solution as aqueous phase, liquid paraffin as oil phase, Span80 and n-butyl alcohol as surfactant, glutaraldehyde solution as crosslink agent. The effect of ratio of diatrizoate meglumine (ratio of W), the ratio of aqueous to organic phase (W/O ratio), the ratio of Span80 to liquid paraffin and the time of crosslink process were optimized by the single factor experiment and response surface experiment. And the optimum prepared microspheres' appearance, distribution, diameter, zeta potential, thermal performance and release properties were measured and their swelling and development properties were observed. The diatrizoate meglumine-loaded chitosan microspheres had a good shape and uniform distribution by light microscope and transmission electron microscope, with average diameters of 1.53 μm and average zeta potential of -19.7 mV; the microspheres had a good sustained release property. And the swelling property of the diatrizoate megiumine-loaded chitosan microspheres varied in different conditions and developed clearly under the ultrasonic imaging technique. This study prepares a kind of diatrizoate meglumine-loaded chitosan microspheres, who can make the microspheres, develop and dredge the obstruction at the same time.