The Effect of Mutagenesis on the Production of Mycelia Growth of Auricularia Species in Nigeria

Abikoye, E. T., Oloke, J. K., Okorie, P. C., Lawal, A. K., Anibaba, A. O., Oluwawole, O. F., Odunuga, A. A., Ajao, M. O., Adelaja, O. D., Okeowo, O. B., Ngajo, A.
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Abstract

Auricularia species is a jelly-like edible mushroom belonging to the class Basidiomycete, Family Auriculariacea. It has many medicinal properties and is widely consumed in Nigeria. Not much research effort appears to have been conducted on its domestication and commercial production in Nigeria. This study evaluated the use of sawdust and rice bran as potential substrates for its cultivation. Two strains of Auricularia species were collected from the wild and identified as Auricularia polytricha and Auricularia subglabra by genetic DNA extraction. Pure cultures of the species were screened for strain improvement by mutagenesis using UV light and ethyl methyl sulfonate mutagens. Spawn of the wild species and the mutants were produced using sorghum grain. The produced spawn was used for the production of the mushroom fruit bodies using sawdust supplemented with rice bran. The biological efficiencies of both wild and mutant were 197% and 90% respectively. A combination of sawdust and rice bran supported the growth of both species of Auricularia.  The Rate of spawn running during spawn production was also negatively affected by mutagenesis in mutants.
诱变对尼日利亚黑木耳菌丝体产量的影响
木耳是一种果冻状的食用菌,属于木耳科担子菌纲。它有许多药用特性,在尼日利亚被广泛消费。在尼日利亚,似乎没有对其驯化和商业化生产进行太多的研究。本研究对木屑和米糠作为其潜在的栽培基质进行了评价。从野外采集到2株木耳,经基因DNA提取鉴定为多毛木耳(Auricularia polytricha)和亚光木耳(Auricularia subglabra)。利用紫外诱变剂和甲基磺酸乙酯诱变剂对菌株进行纯培养。野生种和突变体的卵是用高粱籽粒生产的。将所产菌种用木屑加米糠生产菌体。野生型和突变型的生物效率分别为197%和90%。木屑和米糠的组合支持两种木耳的生长。在产卵过程中,突变体的产卵率也受到诱变的负面影响。
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